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CRIMIC CR71220

Gene Culd

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:8352355..8356296	66C8-66C8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCATTGTCTCTGCGAGACGC	GGCAAATGCTACACGTATAACGG

Images

Construct Design Sequences

sgRNA sequence	GGGCTCAGGATCCTGTTAGAAGG
sgRNA2 sequence	AAAATGGAAGAGTGGACAAGTGG

 

left_200bp	right_200bp
AAATAAATGTATTTTTATACCGGACTCGAGAGGTCTTTAACCCAGTCGAGGGTGGGCTCGTTACGAGGCCCGGTCCACTTGTGCGGCTTCTGATGGCTCTGGGTTTATTGGATTTGCCAGCGAAATGGCCACAGTTGTGTTCTAACACTTGGCGGCACCAGACCGAGACGGTTCAAGCGGAAGGGGCTCAGGATCCTGTT	AAGTGGAACGATAGTATTAAAGCCTGGTGTGATTCTGGTCGGATTCTAGTCACATATAAATAAATAAGCCTGTTTCTTACTCGAATGTTATTAGCTTATTGAAATTCTTAATAAATAATTAACTTCAACTTCATCAAGACTTATCCAAAAGGTCGCAACGTTTGTATTCTTTATAATATGTACCATACCGCAAAAAGAAA

 

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