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CRIMIC CR71222

Gene eEF1alpha2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:31738468..31743306	100D2-100D

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGAGCGAACGTCTTCGTCGAGA	CCCTAGGAGGATTGTTCTTGGAATCG

Images

Construct Design Sequences

sgRNA sequence	AAATGTCTACACATAACGTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGTGAAATTCTCACTCCGAAGAGCTTCCATTTTCAACATGAAAGTGAAAGGCCATTAAAATAAAATAACCCTAGCTAACATATTAATATATGTAGAGCTATTGATTCAAATAAAAATAAATTGGAGTTAGTTCGAATAATATCGCTCCACGTTTCTCTCTCTGTATGCACCCACCCCCATCCAAATGTCTACACATAAC	GTCCGGATATGTAACTTCGTTTCGGTCGCTTCGTTTCCGGTTTCGTTTCAGGCATGGTCGTCAACTTTGCGCCGGTCAACCTGGTCACCGAAGTAAAGTCTGTGGAGATGCACCACGAGGCTCTCACCGAAGCCATGCCCGGCGACAACGTTGGCTTCAACGTGAAGAACGTGTCCGTGAAGGAGCTCCGTCGTGGCTAT

 

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