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CRIMIC CR71223

Gene Klp68D

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:11795614..11798875	68D6-68E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAGGTAATAGCAGTTGATTGCC	GAAGCCGAAATCGATCGGGCAATT

Images

Construct Design Sequences

sgRNA sequence	CACAGTTAACACGGACCTTGTGG
sgRNA2 sequence	TCGACTGGCCAAGTCACTCAAGG

 

left_200bp	right_200bp
TTAGCTCAAGTGGCTGACTAGCTTCAAATTGTGCGTGCAAATCGAGCACAGAAGTGCCCAAATCAGAAGGTTGCGTCAGGAGGGAATTATTGATTTTATTCGTCATTTGTTTTCGGTCGCCACAGCTACTCGACTTTGTTTTTGTTGAGGCGGAGCTGCCTGAGTCACCACCACAGAAAAGAGCACAGTTAACACGGACC	GTGACTTGGCCAGTCGACCCCAACACGCTTCCTTAATTATTTGTATGTTTATTTGTTACTCGACTCGAATTATTGTTATTGATTTAAATGCAAACATACGTACGTTTTGTGTTCGATTAAGTGCAATCTAAGCTTAAGTTAAATCCAGCTGCAACTGTCTATTATTTACTTAGTAAATGATTTTTAGTTAATATTATAAA

 

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