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CRIMIC CR71224

Gene NaPi-III

CG42575

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:11123348..11134663	68A7-68A8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCCACCACAAAAGTAAGGTCCAC	ATAGCAGAGTTGTGTCTTAAACTGCTAA

Images

Construct Design Sequences

sgRNA sequence	AAAACGATGCGATGTAATGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGAGGCAAGCGCAGCGACCGCAAGAAGAGATCCCGACGAAACTAAAGTAGAGACCCCATTGCGAATACCCCAGGCATAACAGATTTGGCCAGACATGTCTTGGCTTTTGTTCCCAAACTTTTCATTTTCTATTATTCTTATTCCTGTATTACCCTCAAGATTAAATGACTTTTTATGATATTCTCAAAAGAATGCCTACA	TTACATCGCATCGTTTTCGACCCTATGAAGTATATTCTCGATCTGAATCACTAGTCGAACTTCAACTGATTTAAGGATGAAGATTCTAGTCATTCCAAAGATTGAAAGTTTTGCTTTTAACGGGTATCTGACAGTCGAGACTTAATGCTTATTGCATTGCATTTTTGATACAAACTTTAGTTTCTGCGGTTAACTACATT

 

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