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CRIMIC CR71226

Gene Sik2

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	X:2055310..2061915	2C8-2C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGAATTTGCTGTGGCAGATCC	GTTCATGTTCAAATCCAGGAGCAG

Images

Construct Design Sequences

sgRNA sequence	AGCTAGTGTTGTTCGTCCAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCCGTTCTACCAATCCCACAGGTTATGGAGACCAAGAATATGATCTACATAGTGTCGGAGTATGCCAGCCAAGGAGAGATCTTCGGTGGGTTGAGGCTTCATTTTAAAAAAAATTTTTTAAACTCGGACTGTAGAGTACAGTACTCGATTTAGTTAGCAGACTTTTGAACCCACATTGCGCACAGACTTGTTGACCTTTG	GACGAACAACACTAGCTTTAAATCTCTCGCATTCTCTTAAGTTGATAAGGGTTTCAATGAGATGGACTTACCTTTATATTTTATCCAGATTACATTGCCAAGTACGGGCGGATGTCCGAGTCGGCGGCGCGCTTTAAGTTCTGGCAGATCATATCGGCGGTGGAGTACTGTCACAAGAAGGGCATAGTGCATCGGGATCT

 

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