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CRIMIC CR71227

Gene Syt14

CG9778

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:4314455..4319069	82A3-82A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCCGCAATCCGTGTGGAGAACA	CAATTGGTACTCGCAAGTAAAGTAAC

Images

Construct Design Sequences

sgRNA sequence	CGAAAGTTGAATGCCTTTCTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGCTGCTCCGCCAAAGCTGGACAAGGAACGGGACAACGTGCTGGTGGTGCCCATGGTCAATGCCTACAGCATTAACAACAACAACAACTCGATAACAACCAGCAATAACCATCCCAGCAGCAACAACAACGATGACGTAAGTTTTACAACATAAGAGGTACTTTAAATACCTATTCCTTTAAAAGAAACTATGCCTAGA	AAGGCATTCAACTTTCGGTCGGACTCGTAAAGTTTACCCAACAAGCATAGTCGAGTCTAACCGACTCTTATGGTCCATATAGTCTGTTAACAATCTTTTTTTGCTTGACAAGTTAAAATTCATTAATGCGTTGTATAAATTCATTAATGCGTTGCATGTGTAAAATAAAAGTTTATTATCTTAACTTTGAAAAATTATGT

 

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