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CRIMIC CR71231

Gene ben

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:13996345..13999805	12D2-12D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCTTTGACTTGCCACGCTCTTC	CGCTATCTCTCCTCCATTTCGC

Images

Construct Design Sequences

sgRNA sequence	CTGGAAACGCGGGTTGAGTCTGG
sgRNA2 sequence	AAGTGGGTGCGTGACATGAACGG

 

left_200bp	right_200bp
TTTCACCGATACATGTAGTTGAGCCCTTATTTAAATTAGTAATTAGTTTAATTTCACAGTTGAAACCACGACTGTAAACATTAGGGATACTAATTTATAGTCTTAATTAACGTTGAGTGGATAGTGGATGATACCGAGGAACTACTTAATTGGTTAACTTCACTCCGATCTCGTTTGCAGCTCAACGAGAAGGTCCAGAC	GAACGGGCGGGTGGGTAAATTGAATACTTTAAAAGCAACCAGAAAAACCTAAAACATACGAAAGAAAACATAAAATAAGAAAAAAGTAAGCAAGCAAACATAAAAAAAAACGATTTAAGAACACATTTTTTTTTCGAACCTTCTGGGGCGGGATATACATATAAAATATTAATATATATATTTTTTTCAACCAATCGATC

 

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