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CRIMIC CR71232

Gene CanB

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:5332499..5334449	4F5-4F5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAGAAGTGGAACATTGGGCAG	TCACCAAGTAAACGCTTGTTGGGG

Images

Construct Design Sequences

sgRNA sequence	GGAGGTCTCGTTACCCATGCTGG
sgRNA2 sequence	ATACTCATACTTGAAACCACCGG

 

left_200bp	right_200bp
TTTTGCACATACCGAATATCCTTCTAATTTTTCTCATTGGTAACTCAAATGTTCCTCAATTCTCTGCTGATATATTTTTTTTTATTATTGAAATTAATTTGGAATCATCTTTTTCTCCGTAGAGCAGAATGCCCTGCCTCCTAACGCTTGCGTTCCATTCGCAGATCTAGACCAGAGAACTAAGTAACTAAAGGCCAGCA	CACCGGCAACGGACCACTCAACCAAAGAACTCATCTATCCCCCTCCTTCCATTCCTCCTTCCATTCGAAATGACATACTTTCGAGGTTCGAACAGCTCTACGATTGGGATTGTTCGCATCATCTTAAACTAGTTTGTACTATATCCTAAACTCTACGTTCAATTCAAGCCTAACACTTAAGTTCATCTCTCGACTAAATG

 

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