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CRIMIC CR71238

Gene CG8197

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:9060461..9061924	45A1-45A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCATTTCAAAGAAGTAATACCACTAAC	CGTGGTTGCAAAGCTTACGC

Images

Construct Design Sequences

sgRNA sequence	TCTTATACACGATAGAGTTCAGG
sgRNA2 sequence	AGGATTACAGAGTGGTGCCTTGG

 

left_200bp	right_200bp
TAGTGGAAGGTGTAAATAGCACACACAAAGTTTGTATTTTACATAGATTCTCTTGACACTTCAGCTAAAAAGTATGCAGCATATAAATAGCCTTTAAATACTTATCGATTTCCTTTCACACACAATCTGCGCTGGCTCAGTTGTCATAAATTCACAATAACTTTTAAATAGTTTAATTATTGATCTTATACACGATAGAG	CCTTGGCGTTAGAAAGTGGATAGAAAGTTGTTAGTTGTGTTTACTCGTAAGTAAATTAAAGTCTATGCCGCTAAAATCAGTTGTGGTAGTACTTCTCAATGATATCGGTTGCAGACGTGTTACGTGGTACTATGATTTTTTAAAGGAAATTTGAATATATATTTTTAAGGGAAAGGTATAAAAATTGTATTTTAAATTAC

 

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