CR71240

CRIMIC CR71240

Gene atl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:24625671..24634714	96A13-96A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGACGTTGACATCTTCTGCGATCG	CACGAGCCACTTACTAATGATGC

Images

Construct Design Sequences

sgRNA sequence	AAGAAAGAATTTCCCAACGGAGG
sgRNA2 sequence	GACCCCGTCCTCTTCCTCCCAGG

left_200bp	right_200bp
GTGCCAAATTTAAATGAACTCACGGCGCAGAGGCTTGCGCAATTAAATGTTGGCCTAGACTCGCAAATTTTCCCTTTGCCGGAAATGGAAAAATCGCTGAAAAACAGAGCAGACATTTTCTCCATACTTATCTAGCTGTCTGTTTATGCTTGCAGTTGACAGGTCGAAATCCAAAAGATTAATAAGAAAGAATTTCCCAA	CCCAGGCGCCAAATAGTCGCAAGAAGCGCAAAGCGAAGCGCAATTAGGTGTCCATGGACCAAGTAGAATGGTTCGATTTTCGAAAGGACGACTCCCCCTCTAATTTGTATAAATCTAAACCCTTCTATAGTGCGTGAGAACCGGAAACACAAACGTCTAGCTATTAATTGCCCTTAAAGGAAGTTGTTTTTATATTGCAT