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CRIMIC CR71241

Gene Bruce

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:10312039..10331353	86A7-86A8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGACAACGGAAGCACGGTCAA	CGCAACTAGACCGGTAGAAATGCT

Images

Construct Design Sequences

sgRNA sequence	GCAATATATCAATAGTCTTCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGTCCTGGACGCCAATTCAGGAGTGATTCTGCAGACCTATCACCTCAGTAGCAATGGTAAGATTTCCGAGGAAAAAAAAATCACACCTATGCACACACGAATGGGACACACTCACTCACACTGGCGCACGCAAATGTCAATACAGTGGAAAACTGCGAATTAGAACCCACACAGCAAAAAATAGCAATATATCAATAGTC	TTCTGGTTCATCGAATATCTGAATTGTCTGAATCATATCTATAGAAGAACGATGTAACACAGATTTCAAATTGTTGCTAAACATTTTGGTCTTAAACAGAAACATTAACAAATTTAAAAAATACAATATTAAATTTTTATCTATTATTGAAATTTTCAATTTAAATTTGATTTAAGAGTTCCATTGAAAATGCTAATGTA

 

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