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CRIMIC CR71242

Gene Det

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:17149669..17150455	89F1-89F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	GACGACTGTTGGCCATAACAG	GTGTGCTCTCAGGAGGATTCAGTT

Images

Construct Design Sequences

sgRNA sequence	GTTGCTGTGCCTTCAAAAACAGG
sgRNA2 sequence	TAATTTATAAATACATTCGATGG

 

left_200bp	right_200bp
CGCTCACGTTACATTAAACATTGTTTAAAAGTAACCGTTTTAATGGAGCAAAGGAACATTTTAAGAGTTTTATCCGTACTGTAGAAGTCTCTATGAGTGGCATAAAACATTCTATTATATTTTTAAATTCTTACTCGGATATCGATAATAACCGGGCTTACAAACTATCGATTATTTTGGTTTTATATGGCAACCCTGTT	AATGTATTTATAAATTAAACTGTAATAGGATTTTTTTTTAATTGCTTATTTTAAACCAATCTGTCTCGTGTTCAATTTAAAAACTTCAAGGTCGTATATGGTCATACTGAGTCTTCTATGTGGAACAGCTGATTAAAACCAAATCCAATGCAGCTTTTAAATCCTGCGATTTGTTGTCCTGTTCCGCTGAAGCAAACGTT

 

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