CR71243

CRIMIC CR71243

Gene CG10321

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:21634679..21638399	57F5-57F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTATGAACGGCAAGGAATGGCT	CGACACGTGGCAATTGGGAATCAA

Images

Construct Design Sequences

sgRNA sequence	TCATCAATTCATTCATCTCATGG
sgRNA2 sequence	AAATGCCAGTCTGTATTCCCCGG

left_200bp	right_200bp
CATAAGAAGTTTTAAGTATTAAAAAGAAATCTAGAAATCTGAGGAAATAAACAAAATGATTTCCGACCGGCATGCGTTAGGGATTTCTGTCTTTATACCACGTTGGCAGCGCCATTCACAACACGCCTATCATCTCACTCACACTCGCACACAGAGAAAGACAGATAGAGGCAGAAGTTCAGTTCATCAATTCATTCATC	AATACAGACTGGCATTTTAGGCTAAACGTATTTTGGCGTTGGGTTTTCATTGTTGGGCTTTGAAATAAGTGAATTTGTTTTAACTGAACAACAAAATAAATTAACAAACCTTCTATGAAACCATAATTTGTCTTTCTTGTACGTCCCATCTGGCCGGTAATATTTATTTTTAAGCATGTAATCCAATTCGCTGGCTCTGT