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CRIMIC CR71244

Gene CG44002

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:3169820..3171092	23D4-23D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCATGCTAAGAATTACGGCAAGG	GTTTCTCTACACCTAAGCTAACACTG

Images

Construct Design Sequences

sgRNA sequence	CTTTGATCGATTTGTATAGGCGG
sgRNA2 sequence	CTTTCATATAAATATTCTATTGG

 

left_200bp	right_200bp
GGTAACGTAACATACAAATTCTTTTGCGCCTAACAGCCCACTGTTGTGGGAACAGGGTGATCATTCCACTAGCAAACTGAATGCTATGCCAAAATGGTAAACAAACTATTCCCTATAATAAATAAACCGTCTCATTTAATTTGAAAATATTTAAAATACCACTACATATTTATTTGTAATTACCTTTGATCGATTTGTAT	GAATATTTATATGAAAGCAAACACACAAATCTAGCACAACATTCTTTGAAACATTATGAAAAAGGATCAAATTAATAACCTTAAAATTGTTCTTTTGGGCAAAAAGTTAAAAACAAAAAAGTAACTCTTGTCATCGCAGTCGGTAGGATTCGAACCTACGCTCCCAGAGGGAATCTGATTTCTAGTCAGACGCCTTAACC

 

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