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CRIMIC CR71246

Gene Plzf

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:12046711..12048918	33B11-33B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGCACGAACTGCATTTTAGG	TCAAGAACCTGCCACAATATACCAC

Images

Construct Design Sequences

sgRNA sequence	GTTCTTAAGCTTTAAGGAAAAGG
sgRNA2 sequence	TTCTCTGAGAAGTTATGAATGGG

 

left_200bp	right_200bp
CTGTGACAGAACTACTGAATCTCTACCAGTTGCAGCCCTTGGGCGAGGCGAAGGAAGCAACGGAGATCCCAGCACCTGGAGAGGCGCAACCAAATCCAGATCCAGAAAAGAAAGCGGAGGCTGTGTTTGAGAATCGACAAAGTTACTTCAAGGTGGATAATATGTCATTGAGCCACTGCCGTAACTTATATTCGCCTTTT	CATAACTTCTCAGAGAAAGAGATTTGTGAGGCAAAGGTCAATAGTAAATATTTAGAATAACTATGTTGAAAAGAGACTGTATCAGCAAGTTTAGAACATTTGCTATATTTACAAAATGAGACGTGGGATCTCTCGTTCTGAGTTCTAGTACAACAAAATTGTATCTAGAATATTCACGGAAACCCAGTTTCTTTTTAAAG

 

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