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CRIMIC CR71247

Gene CG31365

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:23123962..23126422	94E1-94E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGACGTAGCCACGTAACAACGAA	CAGAGCTGTTCCAAATGTTCCGAT

Images

Construct Design Sequences

sgRNA sequence	AAATCAACGTATTTACTCGGTGG
sgRNA2 sequence	AGCTTGAAACTCGTTCGTCTCGG

 

left_200bp	right_200bp
ATTCGGTATGCATTGGATTTGTATGAAAATCAATACCTTTAAACCTTACATTAATAAATTAAGTTATGATACGTTTATGGGCCGTATATATCAAATTGTTTATTTACGAACAGTGTGACTGTATTGCTTTCGATTCGAGAGGAGGTATTTTTAGCGAACCTGTCTGCGGTCACATTGCTTGCGATGCAGAGACGCCACCG	CGAACGAGTTTCAAGCTGTGGCCTAGAATAGTCCCTGGACATGTTTTACCAATACACCGCAGATTGGACCACCATGTTTTACACCTGAAGAGACCAACTTTGATTGATCTGAATATTTTACAACTTTATTCGCGAACCTAGAGTGCTCACAACAGTTGGCACCAAAAATCATCAACATGATAGCGTTAAAACATTTAGAA

 

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