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CRIMIC CR71249

Gene CG12605

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:3954251..3964771	64A1-64A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACAGGGTCTTTGTGCACTGTCTT	TTACGCCTCCCAATCTTCGTTCAG

Images

Construct Design Sequences

sgRNA sequence	CAACCGCAGCAATACTCTCAAGG
sgRNA2 sequence	CAGGACATTGTGGTGGCCTAGGG

 

left_200bp	right_200bp
ACAACGAAAGCCAGATACCTTGAACTGCGGAGATAGCCGGGGGTACAATTTAAATAAGCTGCCAGGCGGATGTGGTAGGATCGCTGGTGGGCAGGATCTTGCGGGGGGCAGAAGGATGCCAGCCAAAGGACGCAATATTGCAACATCCGCAAATTCGGGGACACGGAATCAAAGCTGAAGGAGCAACCGCAGCAATACTC	CTAGGGACAATCGACCTAGGGAACTCTCCTGCCCGCAACTAAGATACTTTATACCAGAACCCTATAAGCAATACTCGAGCACCCTTATCAAATTAATACTAAAATGAATTAACAAAACCAAATGAGAATTATAAACAAACAAAATCTAGTCTAGAAATCGGCTTACTAGCGGAGTAGTTCTTTAAATGGCAGATGATCAC

 

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