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CRIMIC CR71251

Gene Kr-h1

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:6082603..6096498	26B5-26B5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCACTCGAAGAACCAAGATC	GATGAATGTCTTCTCGCACACG

Images

Construct Design Sequences

sgRNA sequence	TATTAATATCACTGATATGGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCCGTCTCAACCGCGGCCATCGAGCTCAATGATGCCGGTCTGCCGGTGGGCATTCCCAAGAGCCCCACCATCAAGCCGCTGGCCAACGTGGCCGCCGGAGCAGATCCCTATCAGTGCAATGTTTGCCAGAAAACATTCGCCGTACCCGCCAGGCTGGTAAGTGTCACGAAAACAGAGATTTGAAATATTTGGACCACCA	TATCAGTGATATTAATAAGTAATATATCTCCTTCGCTTTTAGATCCGCCACTACCGCACCCACACTGGTGAACGGCCATTCGAGTGCGAGTTCTGCCACAAGCTGTTCAGCGTGAAGGAGAACCTCCAGGTGCACCGGCGCATCCACACGAAGGAGCGTCCGTACAAGTGTGACGTCTGTGGACGGGCATTCGAACACTC

 

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