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CRIMIC CR71252

Gene brk

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:7307939..7311132	7B1-7B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGAAGAAGCAACAGCACGCAGACA	CAAATTCAATTGTTTCGCCTTGTTTCAC

Images

Construct Design Sequences

sgRNA sequence	CACAAAACTGACCAGAATCTTGG
sgRNA2 sequence	ATATGGCTTAGGGAACTAAAAGG

 

left_200bp	right_200bp
GTCGCAGTCGACGTCGCTGCTTTGGGGACAGAGCTGCCAGATCGCGAGTTATGAAAGCGCAAGCGTCGCACGCAAGTAGCAACAACAGCTACAACAAAAGCACCTAAAACTTACTACCTACTACTACTGCAAAACACACTTGTTTGACTATTATTTCGCTACATTAAAATCAACAAAAATACACACAAAACTGACCAGAA	AGTTCCCTAAGCCATATTTAAGCTTAATAACGTTTTTTTTAGCCCTACCACCTAAGTTTACCTTAATAAAAAATTGTATATCTAAGCGCAAACATATATCTTCCAAGCATATCAGCAAAAAAAACCCCAAACCCTAGGTATAAACCCTTCTTTTTTTTTTGGTTACACACTAAGAACAAATTAAGCTTAAGTTCAGATTT

 

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