CR71254

CRIMIC CR71254

Gene CG10466

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0CAAGTTTAAACAATGATTTCAGG	2L:19792104..19792782	38A3-38A3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCTAACAGCAAGCAGTCACATCG	GCTACAAGCGAAGGATTCTGAAGG

Images

Construct Design Sequences

sgRNA sequence	CAAGTTTAAACAATGATTTCAGG
sgRNA2 sequence	AAATTATAGATGATAAGATAAGG

left_200bp	right_200bp
GCGGAAAGAAGAGCTGGCACGACATGTACAAGGACTCAGCCTGGATTTTCGTCGCAGGATTTCCGTATACCCTAACTGAGGGCGACTTGGTTTGCGTATTCTCGCAGTACGGCGAAGTGGTCAACATCAATCTAATTCGAGATTCAAAAACGGGAAAGTCCAAGGTAATGCTTAGTTACCCTTTCAAGAAAGTTCCTGAA	ATAAGGTTAGCTTAAATATAGCAAAGCATGCAAACAGAATTTTTATATTAACTTAAATTTTTTAGTTATTAAACGCTAAATTATACTACTACATTCTTTTTTAAAGCCAGTAATTTAAGGTGCAAGAATGAATTGGCTTTCCAACCTCTCATTCGCCATCAGCGCTCGCAAAGGAGACGTCATCATCATCATCATCCTCA