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CRIMIC CR71255

Gene Poxn

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:15826693..15835055	52C7-52C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCATCAGATGCCATCTCCAGTC	AAGCATCAGATGCCATCTCCAGTC

Images

Construct Design Sequences

sgRNA sequence	AGACAAATGGAGCTTATAGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGGTTATTACTGGCGCACTTTTGCCGCCTGCGTTTATGACCGGGGGTTGATAGGAGGGGGGAAAGGTGAGAAAGAGATTAGCCATGTCAACAGGGCGGTTGCCAAACTGCGGGTATTTGGCCCAGACGCGTGTGCCCACTATAAATCTTAAAGACTTTATTCGCTTAATTGGGAGGCTTAGGCGCTTTTAATTCCCACT	ATAAGCTCCATTTGTCTAGCAAAATTTACTTCCAAATATATGGCTTAACACTAACAGTTTAAGACAATACTTTCTAAGTATCTGTAAAGAAGCTGCTAATCTTGTTAATCTTGTTCAAAGCGAACTTAAAGATGGTTCTTTTCAAAGCCTTTAAATGTATATTAAATGGGTGAGGAGTACGTATTGGGACCAGGCAAAAA

 

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