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CRIMIC CR71256

Gene Rab26

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3L:21318774..21335027	78C7-78C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCCCATGAATAGAGCTGGTATT	AATAGCTCGGCACATAGCGACCAT

Images

Construct Design Sequences

sgRNA sequence	TGAAAGGGGGAAGTGCCTTACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTTGAGTAGGTCTGCTCACCGTTTTTATGTAACATTTGTGCTGGGTGACCAGCTGCTAGCTAAAAAATTCCCTGGAAAATCAGCAATTGCATGTTTGCATAACCAAACCAACTTTGCGTATTTTAGTCGAGCCAACAGCAGCTATCAGCAGTAGCCTTTAAAAGCATTTTAGGAGGTAAACTGAAAGGGGGAAGTGCC	TTACGGTAAGGATATCCCGAAACTGAGAGCATTCAACACTCCCGCCCGACTGTCATGTAAAATTCATAAGCAGTTCATTAAATATTTAGCTGGAAACATTAGGTTGCGGCATAAATTATTATGTGAAAATCGAACCACTACAATGTACTTAACAATTCACGTATCACATGGTTTTCTTTGGTTTTTCTTGGCTTTCCCCG

 

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