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CRIMIC CR71258

Gene TbCMF46

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:9922737..9924865	30F1-30F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTACATCACACGGATTGAGAATC	CTTGGAACAGGGACAGCACTTAC

Images

Construct Design Sequences

sgRNA sequence	AATTTCGACACAATCTAAAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGGTCAAGCTGGAGAAGCTATCCCTGTTTAGCAATCGCATCAGGAAAATCGAAAACATACATACTTTGCAGAATCTTGTTATCCTTAGCATTGGAAACAATCTCATCGATACGGTAGAAGGCGTAAGGTTTTTTTCTATTTCATTTCGATAATACTATAAATTTCTTTTTCGGAAACACTAAATTAAAAATGGCCCCTTT	TAGATTGTGTCGAAATTAAAAAGTTCATAAATTAAAGTATGGTTTTTCATCCAGATCGAACGATTACGGTTTGTGAGCAGCCTGAAAGTCCTTAATTTGGAGGGCAATCCCATAGCCAAGCAGCCAGATTTCCCATTGTCCCTCTATGTGATTGCCATTCTACCCCAGCTGAACTACTACGAATACGTTTTCATCAAGAC

 

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