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CRIMIC CR71259

Gene CG13198

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:11654898..11655762	48B5-48B5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCAGATTGCAGTGCTTTCAAC	GCTGATCTTTGTGAAATCCAGGG

Images

Construct Design Sequences

sgRNA sequence	CTATCAGTCTAAAGTTGTAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAACATACTCTTTGATTTCTGCAAACTAATGGCCAGCAGGAATTACGATTTGTCCTTTGAACGATTCATCTTTGATGCTATTCGCAAGCAGAGTAACTTTAATCAAACGTGTCCTTGGAAGGAGGTAAGTGAATAAGGAATTCTTTGTAATCTGGCACATCTTTTTTTGAAGAACTAAAGATTCTATCAGTCTAAAGTTG	TAATGGTATTTCGATAAGAGGTTAATGTTTTAGTTTCATCCGGCTTTTAACGCTTCATTGCAGAACAGAAATTTTTAAAAATCACGAGTGTCTGCGGTTAACTGCATTGTAATGAATATGAATAGAATTTACTCGACATAATCGACTTCCCTTTGACTTGCGCCCTTTATCACTCCATATTGTTCCAGAATCACATGACG

 

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