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CRIMIC CR71260

Gene Tsp33B

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:11791025..11792365	33A1-33A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCCAACTGATCAAGCTCCAAAA	GTCTGCTTGAAGCCTTTCTTGGCA

Images

Construct Design Sequences

sgRNA sequence	ACTTTGGATCGATGCCCACGAGG
sgRNA2 sequence	GAAATGGACGACGAACCGTGTGG

 

left_200bp	right_200bp
GATGTAAATATACATATTTGTAAAGCGATTTTTCAATGCATTATTGTTTTTACTGCCTTTAGGGATAAAAAAAATTAAATTCAAGTTGTAGTACACTTTAAAATTCAAACACCCAAGTCCTTGCAATCAGCGTCAATCCAAATGAAATTCGAGTTCAGAGCTAAAACCTCGTCGACAAGCCAAACTTTGGATCGATGCCC	GTGTGGATACTAATATTGTGCTTAATTTTACTTTTATATTTTATATTAATTTAATTAAATTTACGAATTTGAGTTAACTTCATATCCAATCGGTGAACGCAATATATTATTTATTGGATATTATAGGATACTTATTACATGGATGTTGTAACATTGGATGTTAAATGGATTAACGGCGCATCCTCTTGGATTTCTTCAAA

 

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