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CRIMIC CR71262

Gene CG32832

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:16878326..16879290	36B3-36B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	TATGACTGCCTTTGAATGGGGAGC	CGAGCAGAAGCTATACGATGATG

Images

Construct Design Sequences

sgRNA sequence	AATTTAAAAGCCAAAGTTCCAGG
sgRNA2 sequence	CAATCAAATCAGGAGATGATTGG

 

left_200bp	right_200bp
GTTATCGCACTAATGGATATATCGATAGTTAGCTCACATCTCTACATTGCCGCGCATATTTGAAAAGCTTTGTATGTGAATCGAATGTTCCAAATTGAAAAGTAACCTGTTAACACTTATATGAAAATAAGTCAAGATACATATACCTTAGTTCACTTTGCCTTGGTTATCACTTCAAAATTCTTTTCTTTTTTCCTGGA	GATTGGTAGCAAGAAAATCAATTTATTGAAAGTTGGCGTTATTTGAAATCATCTGAACTTAAAGTCGCATTAACCGAAAGTTAAAGTCGTTATAGACTGAAAAACGGCTGTTTAGTTAATCAAAAAAGATATTACAATCTATAAAGACAGTTCTGTGCCAATAAATGCTTACCATATTGAATTACTCAATGAGCAAATCC

 

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