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CRIMIC CR71265

Gene lok

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:20061422..20064381	38B2-38B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGATCCTCACGCTCAACG	CTGCTTGTCGATCGCTTACC

Images

Construct Design Sequences

sgRNA sequence	TTTGTTGAGATTATCAAAAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCCTGACGCGCATTTCCAAAGTGCATTTTATCATAAAGCGGGCAAACTGCGAACTTACCAATCCAGTTTATATTCAGGTAGGTGGAAGTTCCAAACCCAAAAGCTCTGAAGGAAGCCTTGAAAAAAAAGATAGCAAATTCAGAACCCACAAGAGCAGAATTCAAATTAAGTTTTATTTATGTTTTGTTGAGATTATCAA	AAAAGGTAACTTCAATATTGAATATTTCCCACAATTAATTTACAAAAAGAAGTTACTATTCCGTATAATTCCGTATATTATTAAAATGTAGTAACCTTGTTTCCGCCCATTTCGCAGGATCTCTCACGCAACGGGACGTTTGTGAATAACGAGAAAATTGGCACAAACAGGATGCGAATCCTAAAGAATGACGACGTAAT

 

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