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CRIMIC CR71270

Gene CG31200

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:20744234..20745338	92F10-92F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTTTGACTCTGGCTTGGCTAG	CACACAGAAGTCCTTGTCGTTGC

Images

Construct Design Sequences

sgRNA sequence	TGTTATTAATTATTAACTTGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTTATTAAAAACGCTATATACGTTTTCAGCATTGACGATGGCAGAATTTCGATGCGGTCGTCTGGATGAAAAGCTATTGTACACCACCAACGAGCAGGCGGAACCCTCCGAGAATCCCTGGGTGGGCATTCTGCTGGAGGATCAGGGTCAGTTAACTTTAGAATTGTTAACTATGAAAAGCCCCCTTCTAAAGCCCCAA	GTTAATAATTAATAACATGACCTATGCTTTTGCTAATGCCCTTTGTGTCCACCTTCGATGATTTTCTAGGAAATCGGTACGAGAATACAAGGTGCTCCGTAGTTATTATTAACGAGCTGCACGTCCTCAGCACTGCGACGTGCGTAAAGCGTTTTAGCCAGCGATCCGGAGACACCAAAGCCGTGGCAATGTTAGGCGTT

 

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