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CRIMIC CR71271

Gene ap

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:5706202..5727525	41F8-41F8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCCTAGTCGGAGCATTGTTCCTA	ACTTCATGGTACGCAGCTGGTGAT

Images

Construct Design Sequences

sgRNA sequence	TAGCTACAGCTCGGCAGATCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTGCCCGCGTCCCACGTAATCAACGGACTGCCGCAGCCAGCTCGTCAAAAGGGCAGGCCCCGCAAGCGCAAGCCCAAGGACATCGAGGCGTTCACCGCTAACATAGGTACGTTGTAAATTTCCAGCTCATTCTCGATCAGGTTGCCGGCCATAAAAGAGGCTACAGGCGGTCGGAGCCTTTATAGCTACAGCTCGGCAG	ATCTGGAACCGCCACAAAATCTGGATACTGGCGCCATTAGAGCTGCAAAAGCATCAACTTTTATGTGTCTGCCTCATAAAATGCGGAAAGTGCTGTAAAATAAAAAAAAAACTACAAGGCTCTCTGGATTGATTGCAGATCTCAACACTGAGTACGTGGACTTTGGCCGAGGCTCGCACCTAAGCTCCTCGTCGCGCACC

 

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