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CRIMIC CR71273

Gene Gmap

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	X:15486922..15511223	13C5-13C7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACAGAACAATTTACAGCTGAGGG	GTGCATGGCTTACTTTCACATCTAC

Images

Construct Design Sequences

sgRNA sequence	TTGGCAAATCAATTTCGACCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATATTCGTCACACCAGCGATAAGCGATCGAAGCTGGCTAATTGCATTCGCCACATGTTCCCACACGATTCCAATTCCAATGACAATTGCCATTCCACCCACTCCATTCCGCCGCCGGCGGAATCGGACCATGCAAATTGCCGTCCATATTTCCGCATGCCTGGCAGTTCGTCTTATCGCCAGTTGGCAAATCAATTTCG	ACCTGGTTTGGATATCGTCACTGGGTGCTCTTGCATAAGAGTCCTTTGCCTGGTCTGCTGGCACCAGTTTGGACTAACTGCAGTTGCTATTTTTGGTTGGAAAAGGGGGGACTCTGTATCAAGTATCAATTAATTAATTTCGAAGCATTAGATTTTAAAAACTAGATTACTTTATTGAGAACTTTCTGCTGGGTGTTATT

 

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