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CRIMIC CR71275

Gene sona

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:23753477..23798395	59F7-60A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTAAGTGTTGATTGCTGTCTGGG	CAGACTCACATTTACAGTTAAGATCGC

Images

Construct Design Sequences

sgRNA sequence	CAGCTGGCTCCTGTTGGCTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGCCCTAGAGCCGGCGATTGCACTCAACTCCAATTGCACTCGAGTGGCCAACTCGACGACGCACCAAAAGAGTAGAGTAGCTGCCTGCGTCTGCGTTTTCCTTTCTCAAGCCTCAGTTTCACTTTCAATGGGGCGACGACGACGGTGCGATGACGGCAAAAGTTGCTCAATGCGACCCCAAACAGCTGGCTCCTGTTGG	CTGTGGTCGAAAACACGTTTCAAATGAAAGTAAAAACTCTTTGGCATTGTGCCGAGTCGAGGCGAGACGAGACGAGCGTCTGAGCGCCAAGAGTCTGGTTTTTTCTTCTGCCGTTTGTTCACCTGCCAACTAACTTTCCGCGGGATTTCAATTATAATAGAATTATCATGCTGGCACCTTACGGCTATGCCCGCCAGTGT

 

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