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CRIMIC CR71276

Gene Atf6

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2R:5144733..5154756	41D4-41E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCAAAACTGCTGGATTAAATTC	CGATACTGCATCATTGAGTTGAAGG

Images

Construct Design Sequences

sgRNA sequence	CAGCAACGGCTCCTGATGGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTTATACAAATTGAATTCTAAAGAGGAAGTCTTAATGAATCTCCTTAACGATAGAAACTTTCAGTCATTCAGTCAATTCCTACAACTCCCGCACAAGCAACTGGGCCCTGTTTACATCTGCTCTTAGACCGCTCGGTGATCCTCCGGAACTCGCATTGATACCATTTCAGTATCCTGAGCTACAGCAACGGCTCCTGAT	GGTGGGGTTGCCTCTACTTTACTAGTGCTGTTTCAAAAGTCATTTAACCTCTTAGTTAGCAACTCCGCAAGCCGAAGCAAATTTTCCGCCCGGTGTTAACTGCAATGAGTTACTTTCCGTCAAAATATGCTGTTATAATGGCAACTTATTTGTTTAATGCTGCTACGACCCACCATTCTGAATTCGGTGTTTCCGAGGTG

 

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