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CRIMIC CR71278

Gene Plc21C

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:305935..355566	21B8-21C1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCGATCAGCTGGCGATTTATGA	GAAAGGTGGAGTGCTTCAGTGCAT

Images

Construct Design Sequences

sgRNA sequence	AAACTTTATCAGGTAATTTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGTAAGCGGTAAGGTGTAAGCTGACTTTCTAGTCAGATGGGTAAATTTGGTAAGATCATAGTTACTGCAAATTTGGTGACAGACCCTGACGAGAGCGAGACTCATTCCTATCACGACTCTGCGGTAGTTTGTCAGGTTATCCTGTCAACAGCAGTGGCAGGCAAAGTTTTCTAGTTCCATGAAAACTTTATCAGGTAAT	TTGAGGGGGATGGAAATGGGTTAGGTGGAATATGTATGTAAAGAGAACTAAGCAGGTACATATTTTATACTATGATTTGCATGTAGCACAGCCAGCAGCGGTTTAAACGTATTTCTATAGTTCGAAGTGTACTCTTCAAGGGAGATGAGCTGACTGTTGGGTGTTCCTCAGCAGCATTAGCATATCCTAACCTGCCAATC

 

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