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CRIMIC CR71280

Gene Ptr

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	2R:6181748..6196320	42A13-42A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCTCCAGAAGAGCACTTG	CGGATACATCAGTCTTAACATCAGTA

Images

Construct Design Sequences

sgRNA sequence	GTATCTACACAGGTCGCTTGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGGATGGAAACGCATGCAGATCTGCGGATCTGGTCGAGAACCCCACTGTGGAAAACTGTAATAAATGGTAACTACACATCTATGTATATCTCTTGAAGTTGCGCGCTCCCATTCGGCGCATCATTGAACTTTGTTTGGATATTCGTCTGCCAATAGGCAGCTAAGATACAAAAACCCGTCGAGAGTGTGGAGGCCACAA	GCGACCTGTGTAGATACACATGCACGCGCTTGCTGTTGGTGCTCAACGGAATTATATAGACCGCATTCAGTCGGTTACGACCAGTTAGCCACAAAGCTTAGTGCGCCGCTTCGATTGCACTTTGGCCAAATGTGCCACAAGAGGCGCGCCAGCTGCAACTTTCCCCTGTCTTTTGGAAATTTGTTTATGGAGCGCAACAG

 

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