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CRIMIC CR71281

Gene ephrin

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	4:571755..580122	102C5-102C		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTATACCATCCATATTGCAATGGGAC	GAACCTGATCGAACTCAAATGCAAG

Images

Construct Design Sequences

sgRNA sequence	CACTGAACGCGTAGGGTTCAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACCCCAAAAACAGAAGACAAATGCACGTTTTCGTAGCAAAGGCATTAAACAATAAAGTCTAGATCAGATAAAAACAACCCAATTTTCCATTTTCAAAATGTTAACTTCAGCATTTTCATAAACTGTTGCTTAAACTATTCTGTGCGAACTATTTCAGCATTTTCCAAATCTAAAATTATATATGATATGTAGTTCCTTGA	ACCCTACGCGTTCAGTGCTCATCGTTACTATGTGTGATGTTTTGCACATTAATTGGCCCAAATTTGGTATTTCGATTTGCGCTTATTTATTGAAAATCGTTTTCTGACTTTCTCAAAACAAAATGTAATTTGATTGCACTCTTATATCCACAGATTTCGGATTGATAACACAGATCATATTATCGATGTTAATAAAGGCA

 

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