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CRIMIC CR71283

Gene CG13293

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	3L:6082863..6092188	65A5-65A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCATTTGGAGTGGTCGCTG	TGTGCGGTTGGACACTGAAACTGT

Images

Construct Design Sequences

sgRNA sequence	AGAACAGGGCATATGTGTAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAGGGCTTCATCAAGGCGGAGGCGGCGGTGGCCGTACTGCACGCCGACTCCAATCCCATCATCCTGTCGCACAGGTGAGTCGTTAGCACAGCCTCTGTCCGTACACGCGTATTTGCCCGTGCTTTCGTCGGCGGAGGAGGAGTTTGGACAGCTTGTGGGTGGCCAAGTGTATAGCCTATATCTGTCCGCATATGCCTCTA	CACATATGCCCTGTTCTGTTTGCGTAGGTATACGAGTTTTGCTTGAGTTCCGTTGCCGTTGATTATGTTTAATTTTTGTGCTTATCACAGAAGACTTGAAACACCAAAGAACACTCACCGTTGTCATTTTGTCACGCACTTAAACCATGATCTCTGTCCACCCCCCCAAAACATCCGTCAATCCAATCACAAGATCCAGG

 

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