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CRIMIC CR71286

Gene scro

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3L:24724162..24758036	80F9-80F9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGTACATATATCAGAGACATAGTTGTGG	ACTGTCAAACCTATTCTATTGCTAAGC

Images

Construct Design Sequences

sgRNA sequence	TTACGCAGACAGATGTGTAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAACAATTATTTGTTAAGTATCTCATTTCAACAATCTCATTTTTTATTGTTTTTAAATTTCGAACCCGCAATTGACGAAAATTACTCTAGCTTACCCAGTTCTTTGGAGGTAGAAAGATATTCATATACATATTCGTTGTGAATTTTGATTTCATTTAGCATTTTATGGTTTGCGACAAGGAATTACGCAGACAGATGTG	TAAAGGCCCATATAATTGGAAATATATCATGCAAAGGTATTGTATACCCATGCAGAGGATATATGGATTTTAGTAAAAAGGTTGTAGGGCGGTGTAGAACACTTCACTTTGCATAACTCTTGTTAAAAATACGGATTGATTAACATCTCATCACTGTGGAAGGCAGTTCTCGTAATGATATATATATGAAAACGGTAACA

 

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