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CRIMIC CR71288

Gene kra

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	3R:5595364..5601136	83B4-83B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAACACAAGTTGAGCTCTTGTTG	CCGTCTGATGAGCTTGACAAATAC

Images

Construct Design Sequences

sgRNA sequence	TATGCATGCAACCACTGCGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTCGTGAAGTGGCTGCATATCATTAGAGCTGACTATAAAGTTTCAAAAATGTAATCGGTGTCAAGAATATGCAATAACAATCAACACATTTACTTGTCTTTGGTTTGTCCTAAACACTAACGTTCTGAAGATTTCTTATGTGTCAACAACTACTCTTTATTCAACACTATATATATATACTATATGCATGCAACCACTG	CGAAGGTCACGCTATTGAATCAAAGTGCATATTATTAGAACCGGTTATGCATCTCAACAACTCAATCGGTGTCAAAATATGCAAAATAAAAAGCCACATTTTACTTGTCTTTATTTTGTAAAATTACTACCGCCAGCAATCTAAAGATTTCTAATGTTGCAACAACAAATACAAAAGCCTCCTGCGTGAGAACACAGTAA

 

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