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CRIMIC CR71291

Gene CG6724

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2L:10768234..10769952	32A5-32A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGTGGTTATGCAATCAGCG	TATCGATGTTCGGCTTCTGGCAAC

Images

Construct Design Sequences

sgRNA sequence	GGTGTTGACCACTAAACTAGAGG
sgRNA2 sequence	CAAATCTCGCAAGGCGCTAGCGG

 

left_200bp	right_200bp
GTAGTAACGGGTGATAACTAGACGATAAGGCATTTCGATAAGAGCACAGCGATAGCAATGCGATAACTCTTATTCCGGCATGCGCTTCGGCGTTTTTTAGTGTTTTTGCATGTATTTGTTGCATTTACCATCGTTCGTTTAAAAACACGCTTCTTTTCAATTTAATTTTACACAACGATCTCCAGAAGGAAGAACCTCTA	TAGCGGAAGAAGCGGAGACGAAGTAATCCCAAAACTTGTAAACTTTTTATAAATACAAAACTAGTTTAAACTTTAAAGTAAATATTGAAAGATTTTACATCTGGGGTGTTAAACTTAAATTATCGAAGTGGGTGAAGCAGAGAGGTATTCAAAGTGTATTATACATGGTGTAAATATTCTTGTTTTTTATTTCATTTACG

 

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