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CRIMIC CR71293

Gene Nep3

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:19961297..19969323	19A3-19A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGTATGTAAGAAACCTGTCGGG	ATGGGATACATTTCGTAAGCGTAAGG

Images

Construct Design Sequences

sgRNA sequence	TATTCATGTCAGTTCGCAGAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTGCATCCGTGCAATGCAATTCAGTCGGATAGCCACTTAGTTGTCGTTGGGCTTCAGCTTTATCTTTGTGGCAATGACCCAGAAACGAGTGGCAAATGGGGCCAAAGTGAGCGCTTTTCTCATTTGCTCGAAGGTTGCCAGTTGACAGTGAAAAGTCTGCTCTTATGAGTGGGAGCGCGTTGGTGTGAGCTTTTCCCTCT	GCGAACTGACATGAATACCCTATCTGGTGACAGGGTAACGATGGCTTTGAGAGGCGGTTCGTTGTTCAAATAAATTTAAACTTCATGTGATATTATTCCATAGATATACGAAAGCACTTGACCGTATACAACTGTATCAACAAAATGTCTAAAAGCAAGCTAAATTACTATAGAATTTAAACAGTGTGGTACAATATTTG

 

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