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CRIMIC CR71294

Gene Nep7

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:37954917..37957326	98B6-98B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGGCTAGAGAGATAGCTTGGATG	CCGAGGCTTTAGTCAATCGAATCC

Images

Construct Design Sequences

sgRNA sequence	CGGCCAGTGATCGATCGACCCGG
sgRNA2 sequence	ATCCGAAGGCTGAGAGTTGTCGG

 

left_200bp	right_200bp
CAAAGATGGTGAAAGTGTGCGGAACGACGTCATAGTAATATAATTCATTATAATCTCTGTTGTCCAGTGCTGCTGGTCAACATGATTGCGAAAAAAAAGCACACGCCGATGGGATCGGAGAGATGCTGCTGCTTGCTCAGTTCTCAATCGCGTTCCGGATTAGGCAGTCGAGCTTGTTGGGTTTTTCAAATAATCCGGGT	TGTCGGATGTGGTGACCATAAGTCGTATATAAAGGAAAAGATGTAAAATACTCTAGATGTGCAACTACAAAATAAATGAAAACAGGAAAAATAATGCAAATCGACGATATAGTTTTACACATTTCTGGAATTAAGTTAACTCACTCGACTTTACCCATTTACTCTTAATAGTCTCTTTATTTCTAAGTGGTTTTAATGAG

 

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