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CRIMIC CR71295

Gene Nepl18

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28875621..28877805	98F1-98F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGCATAGTCGGAGGTAGTAGAG	CGCATATATAGCCATGACCGTGTC

Images

Construct Design Sequences

sgRNA sequence	AAAATTAGTTGAGTTTAATTAGG
sgRNA2 sequence	AAAAAACGAATGAACTATATAGG

 

left_200bp	right_200bp
GTCAGTACTCCAACATAAGCTTGACTAGTTTGAAGCGAATTTTTTTTTGAGCACGAATTATATAAGAGTGCAGTTCATATTGTATAAGAGCTGGGAGATAACATGTTGACCTACCCCAGCACCCTGGACATTGTATGAACAAATCCCGCTGATAAGGTGGCCGCCACCGATCGGGAGCAATTGAAAATTAGTTGAGTTTA	TAGTTCATTCGTTTTTTAATTAACTAACTTTGCTTTGATGTTGCCTAACTTGGTAAGCTCAAATGAATGAATGAAAAATAAACAAACAAATATAATAGCATCTTTCAATTGGCATAAATGTAGTTCTGGCATTTATATATAATAAATGCTTAATGTGTATAGAAATGTAAACAAAGTAGCTTTATCAATGAGAGTGCGTA

 

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