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CRIMIC CR71299

Gene Nepl21

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:28833693..28886015	98F1-98F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTGTAGACGAGTTAAGGTCAGCTG	AATCACGGTCAAAGCCAAGTGGAG

Images

Construct Design Sequences

sgRNA sequence	GAGGCACGACAGTCGATCATGGG
sgRNA2 sequence	TTGACCATGTTTACCCTATTTGG

 

left_200bp	right_200bp
AAAAATGATTTGATAAAAATTAGCAAGCTGTTTGCAATCAGCAGTGAGCAATTGGAACTCAGTGTTTGAGCTGAGCACTGTGTCAGAGAGCACCCGATAACCCCAGGGTCGCCTTTTGTTTACCGAGAACCGAGGAGCGGCTTGGCTTTAATGGGGATGGCGATGAACTCATCAGATCAACTGAGGCACGACAGTCGATC	ATTTGGATTTGCTAATATTGAGGGTATTAAAATTATAGTTTCGAGTAAGCGCGAAGAAATTTTGTTGACTTTGGTCAAGAAATTCAAATTTTAAAATCTGTGAAAGAGATAAAATGTAAAAATTGTACTTTAAACTAATAGAAGTGATCAACAAAATCCATATTTAGCTGAGGGTGAATGCTATCTTTAGTTGTCCACTT

 

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