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CRIMIC CR71300

Gene Mtl

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:27636412..27639871	98A13-98A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACTCAGGAAGCAAGTATCCTGA	TTGGCTGCCTTAGCTCATGGCTAT

Images

Construct Design Sequences

sgRNA sequence	CAGTACACAGCTATCGGCACAGG
sgRNA2 sequence	ATTCTCCGTACTCTCGTAAAAGG

 

left_200bp	right_200bp
CATATATGTATTTCTACAGCATTTGTTTCTATTTTAAAGAATTTTTATTAAGGAAGTACGACTGTGAAATAATTTGCGGTCAGTTGGCACTGGCGGGTTTATTGCCTCGAGTAGAACTTAATTAATTGAATTAAGCTGACGAAGACATTCATCTTCCATCTTATTTTGCCCACAGCACACAGACAGTACACAGCTATCGG	AAAAGGCACCGAACCCGGAAAGAAACTCTAAACAAACGATAAATATTGTACGAAATTCTGTGAATCGAAATGTACTGCCATCAAAATTCGTATGAAATTTCTCTTTGTTTGGCTGTTTTTTTCTGCGGATCGTTGCTGTCACACATTTGGCAAACCACGATTCCCTCCCGCAACAACTAAAAAAAACATTGTTAACAAAA

 

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