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CRIMIC CR71303

Gene CG7369

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3L:22741793..22748614	80A1-80A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGTGTCCATATGCAACCGCTCT	GCACAAGGTGTACTTCTCCTTCTG

Images

Construct Design Sequences

sgRNA sequence	CCGGGAGAGCAGCTTATTTGGGG
sgRNA2 sequence	ATATAAATACTGGTCACGTACGG

 

left_200bp	right_200bp
GCGTGCGTCATAGTGTGAGTGTCAGTGCGAAGGTGGTGCGCTGAAACTTGGCGTATTGTGAATTGCCGTGCAAGCACACACACAAACAAAACCGCCCGCCCATCGGAACACCCCTCCCGCTCCAGCAAAAACAACAACGGCGTGTGTTCCACTTTTGACTGTCCTGATTTCGCTGCAGCGCTGCCGGGAGAGCAGCTTAT	GTGACCAGTATTTATATTTATGTATACAATTTTATAGCTAAATGAAATCTTTTTGATATTCCGTAATTACATCCGGGTACATTCTAACGGATTTCATTCTGATTCTGTCTCGAGGCTTTTTGCATCAGCAACATATATGGGCCTACTATCAGTCGCAGGCCATTAATACTTTCGTTTAAGGAAACCAACAGAACAAGTAA

 

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