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CRIMIC CR71304

Gene CG4853

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	2R:17514217..17517445	54C1-54C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACAGTGTTCCTGCAGGTGTGTCT	GTTCGCCACCATTCGTTAGGGAAA

Images

Construct Design Sequences

sgRNA sequence	TGGGGCGGGGTTATCGGGGATGG
sgRNA2 sequence	CGAGGATTGTCGTTAATGATTGG

 

left_200bp	right_200bp
ACCTGTCTTTCTCCCACTGTGTGTGTGCTAATCGAGGTTTCCCCTTGCCCCTTCCTTTCCAGAAATGTACGCGATAGTGGTGCATGTGCATTGCGACTAATCCAAAGAGTCCCCCTGTGGTCATAACGCATTTAACAACCATCGCAGCCAAATAGTCACGTTTCTCCAGAGATCCCCGTGTGTCTGCACTTTCCCCATCC	ATTAACGACAATCCTCGAGATAGTGCAATACAATACAAAACGATGCAATAATTCTCGCACTCTTTCGTTGGCGTTCCATTTTCACATTTTGCATTTGTATCGCGATAAAACAGTAACAATAATAACTCTTACACTTCCACTGTGTTTGTTAATTTGTACTTAAGCTTAGACGACGGTATATATTTATTTGCATTCGAATT

 

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