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CRIMIC CR71305

Gene CG34393

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	2L:3175112..3200496	23D4-23D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGACCATTCAGAGGACACAG	ACCAGGGACTCCAAGCATTC

Images

Construct Design Sequences

sgRNA sequence	GAAAACGAAAATGTGCATGGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGTTGCAAACAAATCCAGAGATTAGCGCTAATTTGATAGGGCTGAATGTGGAATGTGGCCTTTGGTCAGGGAAAACTTTTGAGAGCTGAACAAACACAACAAAAACACCACTAATTGGGGAGATCTTCTAGTTGAGGCAATTCTAAGTGAAAATAAACAAAACACACATGTATAATGTATCAGAAAACGAAAATGTGCA	TGGTGGTTATAAAGTTATAACAAAACAACACATATTCGAATTCATGATTGGATCCTAGGTAGTGAGTGATGAACCTGAGCAGTACTCTACTCTTATATTGTGGCCTACATCTCTTGATTTTCCTTGTAAACTTGTTTTCTTTCTGCTTATTTGCGACTTGCAGGAATTTGTATTCTCGTTTTTATTATCCTGCCGCTTGT

 

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