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CRIMIC CR71306

Gene Hs3st-B

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	X:19248639..19252045	18B5-18B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCTGCGTGACTCTGGTCTACTT	ACACATCGTGCGAAACATATCAAC

Images

Construct Design Sequences

sgRNA sequence	TGGAGCCAACTCAAAATTAGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTCAATGCCTGCCTCCTGGCCAGCATCAGTCGCAGTCTGCAGCAGGTGAATATGTCCACGAATCACTGGACAATGTCTAAGTGTAGGGGCTAAATGTATCCAATATCCAATGCATTTATTCATGAAAACAAACTGATAATTGGAAAATCGAATATAAAGTTTCGCTACACACATGTGTAAAGTGGAGCCAACTCAAAAT	TAGGGGGGGGGGGGGGGTTGTAATAATCCCCAGCGGTTTCAAAACATTTAACAAGCTGTCCAAAAGTGTGCTGTGAAAAGTTTAAGTTTTGTCTTTCGCTGAACTTTGCGATGCTATCTACATACATATATACGATTATATATATGTACATTTATAGTTGCATACTTTTCGGCATACTTACAAGTTGGGCATTTTCATAA

 

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