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CRIMIC CR71307

Gene CG12054

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	3R:31220702..31231259	100B8-100B

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGGCTTATGCTTGTTCTGGCGAT	GACATAACTTAAGGGCAGGCAG

Images

Construct Design Sequences

sgRNA sequence	TAAGTATTCCCTTTCGAGACGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAATCCGTCGCCATTCCGTACCCCGTACGTGCACTCTCGCTATTTACTTATTTCGGCATTTTAATAATAAATTGCCCGGGCTGTGGGCCCCTTGAATGTCTGTGAACTTGTTCCAGAGCAAACACAGAGGTTGGCGTCGAATTGTGGATGCAGGTTTCGCGTTTGGCTGCCCGTATTCCGGTTCGGCTTTCGATCCCGTC	TCGAAAGGGAATACTTAATTGGGTGGTAATCCAGTTTCGGTGACTTTCTAGCAGGGTTCCTAATTACTACCAGTTTTATTCAAAACATTACAATACACATATGGTGTGATTTTAAATTAAATTGCTTTGCAACGAATGCATATTAACATGCCTATTTCATTGCCTTTTCTTGCAGACTATGACCCAAAGGTGGTCGAACA

 

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