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CRIMIC CR71309

Gene Sec61gamma

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:19643327..19644518	18D11-18D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTTTGCCTGTGGACCTTGCACT	ATGTAACGTTGCGTCTCTCAGAG

Images

Construct Design Sequences

sgRNA sequence	TCCATGTCTTGTCAGATATTTGG
sgRNA2 sequence	GTTAACATGCATTATATGTATGG

 

left_200bp	right_200bp
ATGGTGATCTTCAGCCACTGACCCCTGTTGGAGCTGTGATAGCTGGATAACTTGTTGGGCGAATTTGTGGAAATGGGCAACGCGATTTCACCTGTGCCGTCGCCAACCATTTGCCACGTCGCAGCTGACGTTGCTGTGAAAGCGCTGAATACCTTTTACTGATTAGCACTTTCCTTCGCTTTCGTTGCAGAATCCCAAAT	GTATGGACCATGTGCATTCAGCGAATGCCTTTCTAACAAAATTCTACACAAACGTACGAAAACGTATTGTAATGTTTCGATATTCCATTCATGAACAAGTTAGTTAATAAGATTCAAATGTAAAAAGGAATCACCCGTCTTCATAGCATTTGTTTGGGGTGCGCATTCCCGCTAATAAATGTTAAATTGATTTTCCGTGT

 

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